...
- LTs might have worked. Did digest again with different enzymes.
- Did digest with some other interesting ones: LT12 and PT11 and PT12.
Gel
- Transformed TREt:TEVpCofilin LRs
- Picked hEF1a:TEVpCofilin LRs
- Picked pENTR:YFPCofilin
- Picked more LY and PY from new goldengates
- Sent LT and PT for sequencing
...
Notes:
None of the colonies in the new goldengates were blue, so streaked a blue colony in a new GG plate to test it
The lowest band on the LTs seems slightly smaller, but it has bands corresponding to each 3 components. Will find out tomorrow.