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Cloak

Digest of miRNA Generator 1 with ApaLI and XbaI

HL1 Kb | Undig. Prod| ApaLI | XbaI | ApaLI and XbaI A-E

Note: single digest with XbaI should result in a single band at 8 Kbp

 

JA - 7/30

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Transfection notes:

  • 1ug Hef1a:BACE1
  • 1ug TRE:BACE2
  • 2ug Hef1a: rtTA
  • 3ug Hef1a:eBFP
  • 3ug Dummy DNA
  • 500uL diluted OptiMEM-lipofectamine