Gating in FlowJo
- Drag and drop cytometry data file into the interface
- when you double click a particular file, it automatically generates a graph
- you can change the channel that the graph displays
- Forward scatter indicates size
- Side scatter indicates complexity
- height - amplitude
- width - distribution of scattering
- you can change the channel that the graph displays
- you can gate data by clicking the plus sign at the top of the graph window
- gates become a property of a sample
- you can apply gate to other samples by dragging it and dropping it to another cell
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- fca_readfcs();
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