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Gating in FlowJo 

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  • Drag and drop cytometry data file into the interface
  • when you double click a particular file, it automatically generates a graph 
    • you can change the channel that the graph displays 
      • Forward scatter indicates size 
      • Side scatter indicates complexity 
      • height - amplitude 
      • width - distribution of scattering 
  • you can gate data by clicking the plus sign at the top of the graph window
    • gates become a property of a sample 
    • you can apply gate to other samples by dragging it and dropping it to another cell

 

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  • fca_readfcs();

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