Page History

To Do

•  Monday---------------------------------------------------------------------------------------------------------
•  Transform Syk-eYFP
•  miniprep Lyn-STOP
•  Sequence Lyn-STOP, Lyn-TEV, and Syk-eYFP
•  Sunday----------------------------------------------------------------------------------------------------------
•  Pick Lyn-STOP Colonies
•  Sequence Syk-eYFP
•  LR Syk-eYFP
•  Saturday--------------------------------------------------------------------------------------------------------
•  Transform Lyn-STOP
•  Miniprep Lyn-STOP, Lyn-TEV, and Syk-eYFP
•  Friday-------------------------------------------------------------------------------------------------------------
•  Design aBeta binding experiment (CR)
•  Transfect BCR Localization Experiment (CR)
• Experiments
•  Schedule Experiments
•  Pick Lyn-STOP, Lyn-TEV, Lyn-mKate, and Syk-eYFP colonies (CR)
•  miniprep Lyn-STOP
•  sequence Lyn-STOP (AS)
•  Thursday---------------------------------------------------------------------------------------------------------
•  Locate B-Cells (Samira) (CR)
•  Figure out how to fix B-Cells (CR)
•  Seed BCR Localization Test (CR)
•  Organize Plasmids (CR)
•  Pick 10 Lyn-STOP Colonies (AC) (AS)
•  Transform and plate Lyn-mKate, Lyn-TEVp, Syk-eYFP, Lyn-STOP (EE)
•  Miniprep 1-2 and 2-3? (AS)
•  Sequence 1-2 and 2-3? (AS)
•  Order Primers for CD79 eBFP fusions (EE)
•  Design new TCS Fusions
•  Design new TEV Fusions
•  Wednesday-----------------------------------------------------------------------------------------------------
•  Flesh out experiments
•  Organize plasmids (CR)
•  Linker links on wiki
•  Western Blot Protocol (KRB)
•  Design alternative TEV and TCS fusions