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To Do - Saturday--------------------------------------------------------------------------------------------------------
- Transfect aBeta binding test
- miniprep Syk & Lyn
- digest and gel Syk and Lyn
- sequence Syk-eYFP
- Friday-------------------------------------------------------------------------------------------------------------
- Design aBeta binding experiment
- Transfect BCR Localization Experiment
- Pick Lyn-STOP, Lyn-TEV, Lyn-mKate, and Syk-eYFP colonies
- miniprep Lyn-STOP
- sequence Lyn-STOP
- Thursday---------------------------------------------------------------------------------------------------------
- Locate B-Cells (Samira) (CR)
- Figure out how to fix B-Cells (CR)
- Seed BCR Localization Test (CR)
- Organize Plasmids (CR)
- Pick 10 Lyn-STOP Colonies (AC) (AS)
- Transform and plate Lyn-mKate, Lyn-TEVp, Syk-eYFP, Lyn-STOP (EE)
- Miniprep 1-2 and 2-3? (AS)
- Sequence 1-2 and 2-3? (AS)
- Order Primers for CD79 eBFP fusions (EE)
- Design new TCS Fusions
- Design new TEV Fusions
- Wednesday-----------------------------------------------------------------------------------------------------
- Flesh out experiments
- Organize plasmids (CR)
- Linker links on wiki
- Western Blot Protocol (KRB)
- Design alternative TEV and TCS fusions
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