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1. Add Laemmli 2x to lysate (equal volumes of lysate and Laemmli if Laemmli 2x).
2. Transfer to heat block set to 98C and boil for 5 minutes.
3. Store at 4C (reboil if reloading).

Running an SDS-PAGE gel

1. Add gel to gel machine.
2. Add running buffer to cover wells on inner compartment.
3. Add running buffer to the outer compartment.
4. Add protein ladder to first well. How much?
5. Add around 25 uL of lysate (or appropriate volume according to lysate concentration) to each well.
6. Run gel at (voltage/amps).  How long?
7. Wash?
8. Stain with coomassie blue.