Page History

To Do

•  Thursday---------------------------------------------------------------------------------------------------------
•  Locate B-Cells (Samira) (CR)
•  Figure out how to fix B-Cells (CR)
•  Seed BCR Localization Test (CR)
•  Organize Plasmids (CR)
•  Pick 10 Lyn-STOP Colonies (AC) (AS)
•  Transform and plate Lyn-mKate, Lyn-TEVp, Syk-eYFP, Lyn-STOP (EE)
•  Miniprep 1-2 and 2-3? (AS)
•  Sequence 1-2 and 2-3? (AS)
•  Order Primers for CD79 eBFP fusions (EE)
•  Design new TCS Fusions
•  Design new TEV Fusions
•  Wednesday-----------------------------------------------------------------------------------------------------
•  HEK Cell cover slip fixation protocol (CR)
•  Seed for BCR Localization Test (CR)
•  Analyze Lyn Sequences (AS)
•  LR Lyn?
•  GG Syk eYFP
•  pDest 1-2 colonies 2 Amp Chlor plate (CR)
•  pDest 2-3 colonies 4 Amp only plate (CR)
•  Flesh out experiments
•  Organize plasmids (CR)
•  Linker links on wiki
•  Western Blot Protocol (KRB)
•  CD79A & CD79B eBFP fusion designs
•  Design alternative TEV and TCS fusions
•  Call Genewiz (EE)
•  Tuesday----------------------------------------------------------------------------------------------------------
•  Immunostaining Microscopy protocol
•  Immunostaining Flow Cytometry protocol
•  Western Blot protocol
•  Gel extract new Syk-eYFP PCRs
•  Transform TRE Gal4VP16
•  Trasfect for membrane localization test
•  Monday-----------------------------------------------------------------------------------------------------------
•  Double check basic plasmids
•  Flesh out initial experiments (CR, KRB)
•  PCR Screen Syk eYFP (KRB, CR)
•  Friday--------------------------------------------------------------------------------------------------------------
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  •  pDEST 1-2
  •  pDEST 2-3 -> from amp only plate, then sequence
•  Additional parts? -> primers, etc.
•  Sequence pENTR Syk-eYFP
•  Review 3D structure linker information (KRB, CR)
•  Wiki/Design
  •  Update and expand on all experiments
  •  how we chose linker links on wii