| Name | Location | Concentration | | pEXPR: hEF1a-eBFP2 | TC Room | Midi prepped | | pEXPR: hEF1a-Gal4VP16 | Our -20 | Verified (being transformed), growing on a plate | | pEXPR: hEF1a-TAL14 | Our -20 | Mini prepped (unverified, have liquid culture)Growing in midi culture | | pEXPR: TAL14-mKate | TC Room | Midi prepped |
EXPERIMENTS: - hEF1a-eBFP2 & 5xUAS/4xTAL14bs-eYFP
- negative control (minimal yellow)
- hEF1a-eBFP2 & 5xUAS/4xTAL14bs-eYFP & hEF1a-Gal4VP16
- positive control (hopefully linear BFP/YFP)
- hEF1a-eBFP2 & 5xUAS/4xTAL14bs-eYFP& hEF1a-Gal4VP16 & hEF1a-TAL14
- Should be blue (minimal yellow)
|