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Lysis buffer (from Abcam)

pH 8.0

150 mM NaCl

10% NP40 or Triton X-100

50 mM Tris

Running buffer (from Bio-Rad)

pH 8.3

25 mM Tris

192 mM glycine

0.1% SDS

Gel stain (from Abcam)

0.3 M CuCl₂

Gel destaining buffer (from Abcam)

pH 8.0

0.25 M Tris

0.25 M EDTA

Transfer buffer (from Abcam)

48 mM Tris

39 mM glycine

0.04% SDS

20% methanol

Membrane stain (Ponceau Red) - stock (from Abcam)

Dilute 1:10 for washing the membrane

2% Ponceau S

30% trichloroacetic acid

30% sulfosalicylic acid

TBST (Antibody/BSA buffer) (from Abcam)

100 mL TBS 10x (pH 7.6)

• 24.23 g Trizma HCl
• 80.06 g NaCl
• to 1L with dH₂O

900 mL dH₂O

1 mL Tween20

Block in 5% BSA (5g BSA in 100 mL TBST)