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To Do - Wednesday-----------------------------------------------------------------------------------------------------
- HEK Cell cover slip fixation protocol
- Transform for BCR Localization Test
- Analyze Lyn Sequences
- LR Lyn?
- GG Syk eYFP
- pDest 1-2 colonies
- pDest 2-3 colonies
- Flesh out experiments
- Organize plasmids
- Linker links on wiki
- Western Blot Protocol
- CD79A & CD79B eBFP fusion designs
- Design alternative TEV and TCS fusions
- Tuesday----------------------------------------------------------------------------------------------------------
- Immunostaining Microscopy protocol
- Immunostaining Flow Cytometry protocol
- Western Blot protocol
- Gel extract new Syk-eYFP PCRs
- Transform TRE Gal4VP16
- Trasfect for membrane localization test
- Monday-----------------------------------------------------------------------------------------------------------
- Double check basic plasmids
- Flesh out initial experiments (CR, KRB)
- PCR Screen Syk eYFP (KRB, CR)
- Friday--------------------------------------------------------------------------------------------------------------
-
- pDEST 1-2
- pDEST 2-3 -> from amp only plate, then sequence
- Additional parts? -> primers, etc.
- Sequence pENTR Syk-eYFP
- Review 3D structure linker information (KRB, CR)
- Wiki/Design
- Update and expand on all experiments
- how we chose linker links on wii
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