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titleL7ae Repression
Grown in midi culture
NameLocationConcentration
MAV1212-hEF1a-eBFP2Our -20Grown in midi cultureMidi prepped
pEXPR:hEF1a-L7aeOur -20Midi prepped
pEXPR: CAG-2xKturn-eGFP (wt)Our -20Midi prepped
pEXPR: hEF1aCAG-2x KturnexKturn-eGFP (mut)Our -20Grown in midi cultureMidi prepped

 

EXPERIMENTS

  1. hEF1a eBFP2 & hEF1a 2x Kturn eYFP (positive control)
    1. Should be yellow and blue
  2. hEF1a eBFP2 & hEF1a 2x Kturn eYFP & hEF1a-L7ae (negative control)
    1. Should be blue (minimal yellow)

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titleTAL14 Repression
NameLocationConcentration
pEXPR: hEF1a-eBFP2Our -20Grown in midi cultureMidi prepped
pEXPR: hEF1a-Gal4Vp16Gal4VP16Our -20Being LR'dVerified
pEXPR: hEF1a-TAL14Out Our -20Grown in midi cultureMini prepped (unverified, have liquid culture)
pEXPR: 5xUAS/4xTAL14bsTAL14-eYFPWith Brian?mKateOur -20Mini prepped (verified, UAS needs to be added somehow?midi'd)

 

EXPERIMENTS:

  1. hEF1a-eBFP2 & 5xUAS/4xTAL14bs-eYFP
    1. negative control (minimal yellow)
  2. hEF1a-eBFP2 & 5xUAS/4xTAL14bs-eYFP & hEF1a-Gal4VP16
    1. positive control (hopefully linear BFP/YFP)
  3. hEF1a-eBFP2 & 5xUAS/4xTAL14bs-eYFP& hEF1a-Gal4VP16 & hEF1a-TAL14
    1. Should be blue (minimal yellow)

 

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