How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
- Meet w/ Kyle
- FlowJo
- Bright Field Images
...
miRNA Sensors (Theory)
- Troubleshoot
- What's going on with the double input low sensor
- Is there a different GG thermal cycler program for Bbs1?
- Run new transfection plate/protocol by Brian
- Move siRNA into our cryobox
- Grab sequencing primer from Jeremy's bench
...
miRNA Sensors (Lab Work)
- Pick colonies
- pEXPR: MAV1212-hEF1a-TAL14 (Mini)
- Give plates to Brian
- pENTR: TAL14p
- pENTR: TAL21p
- pENTR: Something for yeast for Brian
- Restriction Digest & run gel
- pEXPR: MAV1212-TRE-L7ae (LR)
- pEXPR: MAV1212-TRE-GAL4VP16 (LR)
- Transform
- pEXPR: MAV1212-hEF1a-GAL4VP16
- Golden Gate
- All the sensors
- Split and Seed 4 plates
...
NOTES
We transfected way too much DNA --> new protocol with 1 ug total