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changes.mady.by.user Unknown User (clrichar@mit.edu)

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changes.mady.by.user Unknown User (clrichar@mit.edu)

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title	Setup

Well 1

B-Cells

Well 2

HEK293

Well 3

HEK293

Dummy DNA 1000 ng

Well 4

HEK293

mKate 200 ng

Dummy DNA 800 ng

Well 5

HEK293

Gmab L 200 ng

Gmab H 200 ng

CD79A 200 ng

CD79B 200 ng

mKate 200 ng

Well 6

HEK293

Gmab L 200 ng

Gmab H 200 ng

CD79A 100 ng

CD79B 100 ng

mKate 200 ng

Dummy DNA 200 ng

Well 7

B-Cells

Well 8

HEK293

Well 9

HEK293

Dummy DNA 1000 ng

Well 10

HEK293

mKate 200 ng

Dummy DNA 800 ng

Well 11

HEK293

Gmab L 200 ng

Gmab H 200 ng

CD79A 200 ng

CD79B 200 ng

mKate 200 ng

Well 12

HEK293

Gmab L 200 ng

Gmab H 200 ng

CD79A 100 ng

CD79B 100 ng

mKate 200 ng

Dummy DNA 200 ng

Well 13

B-Cells

Well 14

HEK293

Well 15

HEK293

Dummy DNA 1000 ng

Well 16

HEK293

mKate 200 ng

Dummy DNA 800 ng

Well 17

HEK293

Gmab L 200 ng

Gmab H 200 ng

CD79A 200 ng

CD79B 200 ng

mKate 200 ng

Well 18

HEK293

Gmab L 200 ng

Gmab H 200 ng

CD79A 100 ng

CD79B 100 ng

mKate 200 ng

Dummy DNA 200 ng

Well 19

B-Cells

Well 20

HEK293

Well 21

HEK293

Dummy DNA 1000 ng

Well 22

HEK293

mKate 200 ng

Dummy DNA 800 ng

Well 23

HEK293

Gmab L 200 ng

Gmab H 200 ng

CD79A 200 ng

CD79B 200 ng

mKate 200 ng

Well 24

HEK293

Gmab L 200 ng

Gmab H 200 ng

CD79A 100 ng

CD79B 100 ng

mKate 200 ng

Dummy DNA 200 ng

Expand

title	Expected Results

We expect to see clear yellow fluorescence around the blue stained nuclei indicating BCR localization to the HEK cell membrane. This will be corroborated by similar results with B cells.

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