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titleSetup

Well 1

Just Cells

Well 2

1000 ug Dummy DNA

Well 3

hEF1a: eBFP 250 300 ng

Dummy DNA 750 700 ng

Well 4

UAS: eYFP 250 300 ng

hEF1a: eBFP 250 300 ng

Dummy DNA 500 400 ng

Well 5

hEF1a: Gal4VP16 250 ug300 ng

UAS: eYFP 250 300 ng

hEF1a: eBFP 250 300 ng

Dummy DNA 250 100 ng

 

 Well 6

EMPTY

Well 7

hEF1a: rtTA 250 100 ng

TRE: Gal4VP16 250 Gal4VP16 300 ng

UAS: eYFP 250 eYFP 300 ng

hEF1a: eBFP 250 eBFP 300 ng

0 uM Dox

Well 8

hEF1a: rtTA 250 rtTA 100 ng

TRE: Gal4VP16 250 Gal4VP16 300 ng

UAS: eYFP 250 eYFP 300 ng

hEF1a: eBFP 250 eBFP 300 ng

1X Dox

Well 9

hEF1a: rtTA 250 rtTA 100 ng

TRE: Gal4VP16 250 Gal4VP16 300 ng

UAS: eYFP 250 eYFP 300 ng

hEF1a: eBFP 250 eBFP 300 ng

10X Dox

Well 10

hEF1a: rtTA 250 rtTA 100 ng

TRE: Gal4VP16 250 Gal4VP16 300 ng

UAS: eYFP 250 eYFP 300 ng

hEF1a: eBFP 250 eBFP 300 ng

100X Dox

Well 11

hEF1a: rtTA 250 rtTA 100 ng

TRE: Gal4VP16 250 Gal4VP16 300 ng

UAS: eYFP 250 eYFP 300 ng

hEF1a: eBFP 250 eBFP 300 ng

1000X Dox

Well 12

hEF1a: rtTA 250 rtTA 100 ng

TRE: Gal4VP16 250 Gal4VP16 300 ng

UAS: eYFP 250 eYFP 300 ng

hEF1a: eBFP 250 eBFP 300 ng

2000X Dox
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titleExpected Results

Well 1

No fluorescence

Well 2

No fluorescence

Well 3

Blue Fluorescence

Well 4

Blue Fluorescence

Leaky Yellow?

Well 5

Blue Fluorescence

Yellow Fluorescence

 

 Well 6

EMPTY

Well 7

Blue Fluorescence

Leaky Yellow

 

Well 8

Blue Fluorescence

Yellow Fluorescence

 

Well 9

Blue Fluorescence

Yellow Fluorescence

Well 10

Blue Fluorescence

Yellow Fluorescence

Well 11

Blue Fluorescence

Yellow Fluorescence

Well 12

Blue Fluorescence

Yellow Fluorescence

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