Page History

How To Please Ron/Brain

•  Keep updating the parts tree (include experiments)

miRNA Sensors (Theory)

•  Troubleshoot
  •  Why didn't the oligos nanodrop??
  •  Meet w/ Kyle about FlowJo
  •  Look for the siRNA

miRNA Sensors (Lab Work)

•  Golden Gate
  •  Low Sensor miR-144-miR-181c
•  Pick colonies
  
  •  High Sensor miR-30d
  •  High Sensor miR-146a
  •  High Sensor let-7f
  •  High Sensor miR-125b
  •  Low Sensor miR-144
  •  Low Sensor miR-181c
•  Miniprep
  
  •  pEXPR: MAV1212-TRE-L7ae (LR)
  •  pEXPR: MAV1212-hEF1a-GAL4VP16 (LR)
  •  pEXPR: MAV1212-TRE-GAL4VP16 (LR)
•  Restriction Digest & run gel
  •  pEXPR: MAV1212-TRE-L7ae (LR)
  •  pEXPR: MAV1212-hEF1a-GAL4VP16 (LR)
  •  pEXPR: MAV1212-TRE-GAL4VP16 (LR)
•  Midiprep?
  •  pEXPR: MAV1212-TRE-L7ae (LR)
  •  pEXPR: MAV1212-hEF1a-GAL4VP16 (LR)
  •  pEXPR: MAV1212-TRE-GAL4VP16 (LR)
•  Gateway
  •  pEXPR: MAV1212-hEF1a-TAL14
•  Transform
  •  pENTR: TAL14
  •  pENTR: TAL21
  •  pENTR: Something for yeast for Brian

NOTES

The gel didn't work very well, so we're re-doing it tomorrowImage Removed