Page History

Here's the order for the lanes of the four gels through which we ran our digested GG products:

1kb ladder | Supercoiled ladder | undig. GG donor | undig. GG product | BlpI-digested GG product | x-digested GG product | digested GG products A–J | 1kb ladder (optional)

Note the four gels were arranged in the order:

W Y

X Z

Six of the PCR screen products were identified as potentially being our desired product, and were transformed: XB8, XB4, YA9 ,YB3, YB4, ZB9.

Tasks for Saturday:

1. Stop the LR reaction and store in fridge
2. Miniprep miRNA1 and miRNA2
3. Pick colonies? (maybe not; pick Brian's at least)