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Transformed LR products

 




Digested MAV1212-Hef1a-L7ae with BsmBI in 3.1 Buffer

 

14-7-17

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Miniprep and MidiprepTransformations

MAV1212-Hef1a-Gal4VP16

MAV1212-TRE-Gal4VP16

MAV1212-TRE-L7ae

 

MAV1212-Hef1a-eBFP2-miR181

MAV1212-Hef1a-eBFP2-miR144

MAV1212-Hef1a-L7Ae-miR146

MAV1212-Hef1a-L7Ae-miR7

MAV1212-Hef1a-L7Ae-miR125

MAV1212-Hef1a-L7Ae-miR30

 

 

14-7-18

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DNACon (ng/µL)EnzymeBufferVol DNAMass DNAVol EnzymeVol BufferVol WaterVol Total
Ladder         
MAV1212-Hef1a-Gal4VP16305.5BsaI3.12.4575012.0014.5520
MAV1212-Hef1a-Gal4VP16 control305.5  2.45750  17.5520
MAV1212-TRE-Gal4VP1674.3BsaI3.110.09750126.9120
MAV1212-TRE-Gal4VP16 control74.3  10.09750  9.9120
MAV1212-TRE-L7ae 58.7BsaI3.112.78750124.2220
MAV1212-TRE-L7ae control58.7  12.78750  7.2220
MAV1212-Hef1a-eBFP2-miR181118.6BsaI3.16.327501210.6820
MAV1212-Hef1a-eBFP2-miR181 control118.6  6.32750  13.6820
MAV1212-Hef1a-eBFP2-miR14449.9BsaI3.115.03750121.97020
MAV1212-Hef1a-eBFP2-miR144 control49.9  15.03750  4.9720
Ladder