Page History

To Do

•  Thursday---------------------------------------------------------------------------------------------------------
•  Transform and plate
  •  CD79B+STOP LR
  •  pDEST (1-2, 2-3, 3-4)
•  Make amp/chlor plates (AS)
•  Miniprep (EE)
  •  pEXPR CD79B-TCS-Gal4VP16
  ,
  •  pENTR Syk-eYFP
  & associated
  •  whatever's in the incubator with these
•  Sequence pENTR Syk-eYFP
•  Digest and Gel pEXPR CD79B-TCS-Gal4VP16
•  Add Gmab gel to wiki (KRB)
•  Review 3D structure linker information (KRB, CR)
•  Lyn plates out of incubator
•  GmabH&L=>Verified Plasmids (CR)
•  Pick Colonies from Lyn & associated
•  Wednesday------------------------------------------------------------------------------------------------------
•  Labwork
  •  Midiprep four plasmids
  •  Miniprep Gmab H and Gmab L
  •  Restriction Map H&L
  •  Transform and plate Lyn Golden Gates, Donr 2-3?
  •  Pick colonies (CD79B-TCS-Gal4VP16, Syk-eYFP GG) (also others
  •  LR CD79B-STOP
•  Wiki/Design
  •  Update and expand on all experiments
  •  add gel image to wiki
  •  how we chose linker links on wii