Page History

...

To Do

Wednesday------------------------------------------------------------------------------------------------------
Labwork
- Midiprep four plasmids
- Miniprep Gmab H and Gmab L
- Restriction Map H&L
- Transform and plate Lyn Golden Gates, Donr 2-3?
- Pick colonies (CD79B-TCS-Gal4VP16, Syk-eYFP GG) (also others
- LR CD79B-STOP
Wiki/Design
- Update and expand on all experiments
- add gel image to wiki
- how we chose linker links on wiki
Tuesday-----------------------------------------------------------------------------------------------------------
Miniprep CD79B STOP (AW, AC)
Sequence Midipreps (CR)
Pick/grow up cultures (EE)
- Midipreps
  - pEXPR UAS:eYFP
  - pEXPR 8
  - pEXPR 106
- Minipreps
  - pEXPR Gmab Heavy
  - pEXPR Gmab Light
PCR Lyn (AS)
Gel for Lyn PCR (AS)
Golden Gate Lyn (find AarI) (KRB?)
Pour GG DONR Plates (EE, KRB?)
Digest Gel and Sequence CD79B STOP (CR)
How we chose linker links on the wiki (KRB)
Add gel image to wiki (KRB)
Update and expand on experiments (ALL)
Transform and Plate (AW, KRB)
- pENTR Syk-eYFP (Golden Gate)
- pEXPR CD79B-TCS-G4 (LR)
Midiprep four plasmids (AW, AC)
To bug Brian about
- Tissue culture plasmids (CR)
- How to sequence pEXPRs (KRB)
- CD79B-TCS-Gal4VP16 failures (KRB)
- Super-competent cells? (KRB)
Monday------------------------------------------------------------------------------------------------------------
Golden Gate Syk eYFP

...

Versions Compared