...
- Order LilrB2 and PirB gBlocks
- Simulate Golden Gate on Geneious and make sure it works
- Golden Gate gBlocks into ggDONR
- Transform bacteria with Golden Gate product and plate
- Pick colonies and grow in liquid culture
- Miniprep cells
- Verify miniprep product
- LR into pDEST
- Transform, pick colonies and grow in liquid culture
- Miniprep product
- Midiprep product
- Transfect into HEK293
...