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Comment: Task marked complete

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To Do
Lab work (Can do)(Waiting on parts)(Dependent on other process)ExperimentsPlanning (research?)To Do Tomorrow
 
  •  design expression vectors
  •  make wiki templates
    •  finish homepage
  •  order antibodies
    •  make sure we've got any
      tags, dyes, etc.
    •  anti-IgM
    •  anti-Syk
    •  anti-Lyn
    •  anti-primary (for blots)
    •  anti-pSyk (Tyr323)
  •  update to-do list
    •  add daily status updates
  •  Plan Gibson for BCR Plasmids
  •  Monday------------------------------------------------------------------------------------------------------------
  •  Locate and transform DNA for Tissue Culture (CR)
  •  Transform LRs (KRB)
    •  CD79B-TCS-G4
    •  Gmab Light
    •  Gmab Heavy
  •  Syk stop (KRB)
  •  Send Midiprep DNA for sequencing (AS)
  •  How we chose linker links on Wiki
  •  Update and expand on experiments
  •  Pick Colonies from Transformations
    •  For midiprep
      •  pEXPR 106 = hEF1a-memb-mKate-scfv35-LD0-TEC(L)-G4
      •  pEXPR 8 = TRE-tight:TEVp
      •  UAS: eYFP
    •  For miniprep
      •  pENTR CD79B+stop (1 colony)
  •  Antibodies ordered? (KRB)
  •  Primers ordered? (KRB)
  •  Bring Andrew up to speed (CR)
  •  Talk to LilrB2
  •  PCR Syk eYFP (EE) (AS)

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