| | | - design expression vectors
- make wiki templates
- order antibodies
- make sure we've got any
tags, dyes, etc. - anti-IgM
- anti-Syk
- anti-Lyn
- anti-primary (for blots)
- anti-pSyk (Tyr323)
- update to-do list
- Plan Gibson for BCR Plasmids
| - Monday------------------------------------------------------------------------------------------------------------
- Locate and transform DNA for Tissue Culture
- Send Midiprep DNA for sequencing
- How we chose linker links on Wiki
- Update and expand on experiments
- Pick Colonies from Transformations
- Antibodies ordered?
- Primers ordered?
- Bring Andrew up to speed
- Tissue Culture for Kathryn
- Sunday-------------------------------------------------------------------------------------------------------------
- Transform LRs
- Transform CD79B-STOP DNA from screen
- Transform DNA for Tissue Culture
- Pick CD79B-STOP colonies
- Pick Syk-eYFP and Lyn Colonies
- Saturday----------------------------------------------------------------------------------------------------------
- Transform CD79B-STOPs from screen
- Transform stuff from Syk-eYFP and Lyn Screens
- Design additional alternative cloning primers?????
- Send Mediprep DNA for sequencing
- ------------------------------------------------------------------------------------------------------------------------
- Screen Lyn and Syk-eYFP colonies
- Miniprep/digest verify CD79B-TCS-G4 pEXPR
- Grow up Gmab Heavy miniprep cultures from pENTR plate for sequencing
- LRs: CD79B-TCS-G4 (1), Gmab Light (1), Gmab Heavy (3, 4)
- Grow up midiprep cultures for CD79A, CD79A-TCS-G4, Syk+stop, Syk-TEVp
- Design AarI Primers
- Screen CD79B
- Organize Geneious
- How we chose linker links on Wiki
- Update Experiments list and expand on them
- -----------------------------------------------------------------------------------------------------------------------
- Organize Geneious
- How we chose linker links on Wiki
- Update Experiments list and expand on them
- ----------------------------------------------------------------------------------------------------------------------
- How we chose linker links on Wiki (CR)
- Update experiments list and expand on them (CR, KRB)
- Organize Geneious (CR)
- ---------------------------------------------------------------------------------------------------------------------
- Find all blue plasmids and put in BCR Box (CR)
- LRs
- -----------------------------------------------------------------------
- Email Brian about Pfizer stuff
|