| | | - design expression vectors
- make wiki templates
- order antibodies
- make sure we've got any
tags, dyes, etc. - anti-IgM
- anti-Syk
- anti-Lyn
- anti-primary (for blots)
- anti-pSyk (Tyr323)
- update to-do list
- Plan Gibson for BCR Plasmids
| - Sunday-------------------------------------------------------------------------------------------------------------
- Transform LRs
- Pick CD79B-STOP colonies
- Pick Syk-eYFP and Lyn Colonies
- Saturday----------------------------------------------------------------------------------------------------------
- Miniprep Gmab Heavy
- Sequence Gmab Heavy
- Screen CD79B-STOP
- Transform CD79B-STOPs from screen
- Transform stuff from Syk-eYFP and Lyn Screens
- Run LRs
- Design additional alternative cloning primers?????
- Mediprep green plasmids
- Send Mediprep DNA for sequencing
- ------------------------------------------------------------------------------------------------------------------------
- Screen Lyn and Syk-eYFP colonies
- Miniprep/digest verify CD79B-TCS-G4 pEXPR
- Grow up Gmab Heavy miniprep cultures from pENTR plate for sequencing
- LRs: CD79B-TCS-G4 (1), Gmab Light (1), Gmab Heavy (3, 4)
- Grow up midiprep cultures for CD79A, CD79A-TCS-G4, Syk+stop, Syk-TEVp
- Design AarI Primers
- Design 2 Lyn gBlock (1w/ stop and QX 1W/ fusion)
- Design new gBlock Primers
- Screen CD79B
- Organize Geneious
- How we chose linker links on Wiki
- Update Experiments list and expand on them
- -----------------------------------------------------------------------------------------------------------------------
- Design Digests (EE)
- Transform
- Pick Colonies
- More Sequencing
- Lyn gBlock (CR)
- Redesign Lyn fusions to Q sites (AS)
- Alternative Cloning (KRB)
- Organize Geneious
- How we chose linker links on Wiki
- Update Experiments list and expand on them
- Order DSM Oligos
- Are antibodies ordered?
- Pick colonies
- ----------------------------------------------------------------------------------------------------------------------
- How we chose linker links on Wiki (CR)
- Update experiments list and expand on them (CR, KRB)
- Update various parts pages on wiki with relevant digests (KRB)
- Transform LRs
- Lots of sequencing analysis (AS)
- LRs based on sequencing data
- Order Lyn Gblock W/ Nelson's PCR Flankers (CR)
- Order SDM Oligos for Lyn (EE)
- Organize lab boxes (CR)
- Organize Geneious (CR)
- MAKE SURE ANTIBODIES ARE ORDERED (KRB, CR)
- ---------------------------------------------------------------------------------------------------------------------
-
- anti Syk
- secondary western Ig
- anti IgM
- Design Sequencing Primers (CR)
- Restriction Digests for Orange Plasmids (EE)
- Gel for 7 Plasmids (EE) (AS)
- PCR CD79B's & Gmab (AS)
- Find all blue plasmids and put in BCR Box (CR)
- Golden Gates
- LRs
- -----------------------------------------------------------------------
- Miniprep GG Colonies
- Transform & plate LR plasmids
- Transform & plate GG plasmids
- Email Brian about Pfizer stuff
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