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titleMAV1212-hEF1a-eBFP2
NameLocationConcentration
MAV1212-hEF1a-eBFP2Our -20Miniprepped

 

EXPERIMENTS

  •  MAV1212-hEF1a-eBPF2 (positive control)
    •  Should be blue
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titleL7ae Repression
NameLocationConcentration
MAV1212-hEF1a-eBFP2Our -20Miniprepped
pEXPR:hEF1a-L7aeOur -20Miniprepped
pEXPR: hEF1a-2x Kturn-eYFPOur -20Miniprepped

 

EXPERIMENTS

  1. hEF1a eBFP2 & hEF1a 2x Kturn eYFP (positive control)
    1. Should be yellow and blue
  2. hEF1a eBFP2 & hEF1a 2x Kturn eYFP & hEF1a-L7ae (negative control)
    1. Should be blue (minimal yellow)

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titleTAL14 Repression
NameLocationConcentration
pEXPR: hEF1a-eBFP2Our -20Miniprepped
pEXPR: hEF1a-Gal4Vp16With JinWill meet with Jin
pEXPR: hEF1a-TAL14With BrianpENTR will be LR'd with Hef1a when obtained
pEXPR: 5xUAS/4xTAL14bs-eYFPWith SamiraUAS needs to be added somehow

 

EXPERIMENTS:

  1. hEF1a-eBFP2 & 5xUAS/4xTAL14bs-eYFP
    1. negative control (minimal yellow)
  2. hEF1a-eBFP2 & hEF1a-Gal4VP16 & 5xUAS/4xTAL14bs-eYFP
    1. positive control (hopefully linear BFP/YFP)
  3. hEF1a-eBFP2 & hEF1a-Gal4VP16 & hEF1a-TAL14 & 5xUAS/4xTAL14bs-eYFP
    1. Should be blue (minimal yellow)

 

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