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titleMAV1212-hEF1a-eBFP2

 

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titleL7ae
NameLocationConcentration
pEXPR: Hef1a-eBFP2Our -20Currently being propagatedMiniprepped
pEXPR: Hef1a-L7aeOur -20Currently being propagatedMiniprepped
pEXPR: Hef1a-2x Kturn-eYFPOur -20Currently being propagatedMiniprepped

 

EXPERIMENTS

  1. Hef1a eBFP2 & Hef1a 2x Kturn eYFP (positive control)
    1. Should be yellow and blue
  2. Hef1a eBFP2 & Hef1a 2x Kturn eYFP & Hef1a-L7ae (negative control)
    1. Should be blue (minimal yellow)

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titleTAL14
NameLocationConcentration
pEXPR: Hef1a-eBFP2Our -20Currently being propagatedMiniprepped
pEXPR: Hef1a-Gal4Vp16With JinWill meet with Jin
pEXPR: Hef1a-TAL14With BrianpENTR will be LR'd with Hef1a when obtained
pEXPR: 5xUAS/4xTAL14bs-eYFPWith SamiraUAS needs to be added somehow

 

EXPERIMENTS:

  1. Hef1a-eBFP2 & 5xUAS/4xTAL14bs-eYFP
    1. negative control (minimal yellow)
  2. Hef1a-eBFP2 & Hef1a-Gal4VP16 & 5xUAS/4xTAL14bs-eYFP
    1. positive control (hopefully linear BFP/YFP)
  3. Hef1a-eBFP2 & Hef1a-Gal4VP16 & Hef1a-TAL14 & 5xUAS/4xTAL14bs-eYFP
    1. Should be blue (minimal yellow)

 

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