Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
- More descriptive names
- Include other plasmid
miRNA Sensors (Theory)
- Obtain plasmid maps
- GAL4 (from Brian)
miRNA Sensors (Lab Work)
- Pick colonies & Grow in medium
- GH
- pENTR: L7ae
- Midi prep
- pEXPR: Hef1a-eBFP2
- Mutant Hef1a-2xKturn-eGFP
- Wild Type Hef1a-2xKturn-eGFP
- EF
- Create pENTR: TAL14
- BP Reaction from TAL14
- P4-donor-P1R on Brian's bench
- TAL14-mKate pEST in Brian's fridge
- Transform TAL14
- BP Reaction from TAL14
NOTES
We should sequence the low sensors when they're done