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  • Sequencing results received for pENTR
    • LilrB2 is correct 
    • PirB: Qsite ligation with the C terminus gblock in pENTR
  • New PirB colonies picked 
  • Restriction digest on pEXPR LilrB2 repeated because we suspected quality of digestion
    • HL L1r L1c L2r L2c L3r L3c
      Image Modified



  • Gel verification of LilrB2 and PirB PCR products ran 
    • LilrB2 = 1900 bps

    • PirB = 2500 bps (won't work becasue pENTR is not what we thought it was)