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Make HEK293 expressing LilrB/PirB - (Y)FP YFP fusions. We will test binding beta-amyloid in Experiment B2
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- PCRed extensions onto LilrB2 and PirB
- gel run to verify PCR products
- LilrB2 did not give expected band size
- why?
- PirB did not give expected band size
- pENTR used as template was not right
- LilrB2 did not give expected band size
- gel run to verify PCR products
- PCRed extensions onto BFP YFP
- verification gel to be run
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