Group A: Membrane Localization Localization
In this experiment, we want to test wether or not the receptor proteins, LilrB2 and PirB, localize to the membrane when exogenously expressed in cells.
Group B: Receptor Affinity for Beta-amyloid
In this experiment, we want to test that beta-amyloid binds to the receptor proteins that we have introduced to the cell. We may potentially extend this experiment to trying to characterize the interaction between beta-amyloid and the receptors i.e. try to generate saturation plots and calculate dissociation constants.
Group C: Levels of Endogenous Cofilin Cofilin
comparing endogenous Syk expression to expression of some protein under TRE, since in theory the expression level of TRE:(random protein) should reflect the amount of Syk-TEVp we'll get out from TRE:Syk-TEVp. What Brian suggested was that the "random protein" we use should actually be Syk-TEVp or Syk-eYFP (I guess it removes some of the variables). This way we can compare endogenous and exogenous Syk production directly. In a Western blot, this involves essentially using the antibodies to stain two different proteins - the native Syk and a Syk-fusion. Since Syk and Syk-fusion are different sizes, there should be two different bands on the Western blot afterwards, whose strength we can compare. I'm not sure whether we'll end up using Syk-eYFP or Syk-TEVp for our blots but I can't imagine that it matters all that much. (Maybe we'll try out both?)
Group D: Cofilin Recruitment
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