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Make HEK293 expressing LilrB/PirB - YFP (Y)FP fusions. We will test binding beta-amyloid in Experiment B2
Plan:
Status:
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- PCRed extensions onto LilrB2 and PirB
- gel run to verify PCR products
- LilrB2 did not give expected band size
- why?
- PirB did not give expected band size
- pENTR used as template was not right
- LilrB2 did not give expected band size
- gel run to verify PCR products
- PCRed extensions onto BFP
- verification gel to be run
Experiment B2
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Description:
Testing beta-amyloid binding to receptor proteins using biotinylated oligomerized beta-amyloid. We will test for binding using he biotin-binding protein streptavidin covalently bonded to a fluorophore. We will look for co-localization of the fluorophore and the beta-amyloid.
Plan:
- Oligomerize beta-amyloid monomers
Status:
Not yet started.
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