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- Goal: show A-beta binding to LilrB2/PirB-eYFP.
- Micrograph: fusion protein (yellow), nucleus (blue), antibody (red)
- A-beta has biotin conjugated; need streptavidin/red.
- Streptavidin - Alexa Fluor 594: $185 for 1 mg http://www.lifetechnologies.com/order/catalog/product/S11227
- GFP RabMab (trial size!) rabbit monoclonal chicken polyclonal ICC/WB http://www.abcam.com/cofilingfp-ep6376-antibody-ab134963ab13970.html
- Goal: show LilrB2 and PirB localized to the membrane
- Micrograph: fusion protein (yellow) (or maybe anti-GFP ICC), nucleus (blue), ab (red)
- No LilrB2 proteins verified for ICC. Could ask for an Abreview discount on mouse polyclonal http://www.abcam.com/lilrb2-antibody-ab172538.html
- No PirB abs verified for ICC. Could ask for an Abreview discount on mouse monoclonal http://www.abcam.com/lilrb3-mm0413-9s32-antibody-ab90939.html
- Secondaries: goat/donkey anti-mouse AF594
- Goal: cofilin western blot
- mouse monoclonal ICC/WB http://www.abcam.com/cofilin-antibody-ab42824.html
- rabbit monoclonal ICC/WB (no lead time!) http://www.abcam.com/cofilin-ep6376-antibody-ab134963.html
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