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titleSetup

All combinations of plasmids being tested will be prepared and put into dox ladders

plasmids v  dox>011010010002000
CD79A-stop CD79B-stop      
CD79A-tcs-Gal4VP16 CD79B-stop      
CD79A-stop CD79B-tcs-Gal4VP1      
CD79A-tcs-Gal4VP1 CD79B-tcs-Gal4VP1      

First row is control for basal reporter expression.

 

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titleExpected Results

We should identify what level of Syk-TEVp causes constitutive expression of the reporter and characterize the off behavior of our circuit so we will be able to compare it to the on behavior later.

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