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Testing where in the cell Lyn is |
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To determine if our circuit is assembling the way we expect it to and to see if Lyn recruitment is dependent on activation (which could mean that Lyn recruitment be used as a mechanism for signal transduction). |
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BCR (CD79A/B, IgM heavy, IgM light), Lyn-mKate anti IgM |
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Produce two samples of cells with all parts, anti IgM +/- |
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Lyn should be membrane localized. We will be lucky if Lyn only membrane localizes in anti IgM+ samples. |
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