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This test will test to see if Syk will be properly recruited to the membrane on activation of the BCR. |
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To test that the circuit is working as expected and see if there is an idea level of Syk for on/off |
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BCR (CD79A/B, IgM heavy, IgM light), Lyn, Syk-eYFP, (dox support circuitry) Dox, anti IgM (which forces BCR's to activate) |
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Cells containing all parts will be put into 2 dox ladders (anti IgM +/-) and flow cytometered. |
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The Syk should be more membrane localized in the IgM+ well than the IgM- well. |
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