How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
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miRNA Sensors (Theory)
- Figure out where the Golden Gates went
- Create protocol for siRNA transfection
- Meet with Jin at 3
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miRNA Sensors (Lab Work)
- Transformations
- EF
- GH
- Mutant Hef1a-2xKturn-eGFP
- Wild Type Hef1a-2xKturn-eGFP
- Pick colonies from L7ae and eBFP2
- Miniprep MAV1212
- Digest MAV1212
- Run gel for MAV1212
- Image gel for MAV1212
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NOTES
Jin gave us ideas to optimize our high sensors and experiments