| People to Contact | Questions | Contacted Yet? | |
|---|---|---|---|
Jacob Beal | Had first meeting | ||
Jeremy Gam | Questions | In close contact |
Long Term Goals
miRNA Profile
- Read literature
- Choose profile
Building the Cell Detector Circuit
- Build mathematical model
- Make test bed
- Controlled miRNA expression
- Constructed miRNA sensors
Mini experiments
- Single miRNA detector circuit
Alzheimer's Model
- Agree on a team wide, full Alzheimer's model
Today's Agenda
How To Please Ron/Brain
- Keep updating the parts tree (include experiments)
miRNA Sensors (Theory)
- Figure out where the Golden Gates went
- Create protocol for siRNA transfection
- Meet with Jin at 3
miRNA Sensors (Lab Work)
- Transformations
- EF
- GH
- Mutant Hef1a-2xKturn-eGFP
- Wild Type Hef1a-2xKturn-eGFP
- Pick colonies from L7ae and eBFP2
- Miniprep MAV1212
- Digest MAV1212
- Run gel for MAV1212
- Image gel for MAV1212
NOTES
Jin gave us ideas to optimize our high sensors and experiments