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- Add the provided RNase A solution to Buffer P1, mix, and store at 4C. (One vial of RNase A per bottle of Buffer P1 to give final concentration of 100ug/mL. If you're the one adding, initial top and check box on cap. Buffer P1 will be in the fridge)
- Optional: Add LyseBlue reagent to Buffer P1 P2 at a ratio of 1 to 1000 and mix (If you're the one adding, initial top and check the box on cap)
- Add ethanol (96-100%) to Buffer PE before use (see bottle label for volume) and then check mark on cap.
- Check Buffers P2 and N3 for precipitates, if any redissolve by placing in water bath at 37C Do NOT vortex.
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