Page History

Long Term Goals

miRNA Profile

•  Read literature
•  Choose profile

Building the Cell Detector Circuit

•  Build mathematical model
•  Make test bed
  •  Controlled miRNA expression
  •  Constructed miRNA sensors

Mini experiments

• •  Single miRNA detector circuit

Alzheimer's Model

•  Agree on a team wide, full Alzheimer's model
  

Today's Agenda

How To Please Ron/Brain

•  Keep updating the parts tree (include experiments)

miRNA Sensors (Theory)

•  Update experiments page
  
  •  Obtain plasmid maps
•  Email Jin about TAL14 and miRNA target sites
•  Talk to Lyla/Alexa about transfecting later this week
•  Look for plasmids
  •  pEXPR: UAS-Gal4-TAL14-eYFP
  •  pEXPR: Gal4VP16
  •  pEXPR: Hef1a-TAL14
  •  pEXPR: Hef1a-2x Kturn-eYFP
• Figure out where the Golden Gates went
•   
•   

miRNA Sensors (Lab Work)

•  Transformations Low Sensor Golden Gates
  •  pEXPR: Hef1a-eBFP2 (186 ng/uL)
  •  pEXPR: Hef1a-L7ae (96 ng/uL)
   Pick red colonies and grow in medium!
•  Find concentrations of eBFP2 and L7ae

NOTES

Nelson doesn't know where it is, but he needs it as well, so he's on the hunt

If we're ready, we can transfect very early next week