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titleL7ae
 
NameLocationConcentration
 
pEXPR: Hef1a-eBFP2
- in freezer
Our -20Currently being propagated
pEXPR: Hef1a-L7ae
- in freezer 
Our -20Currently being propagated
pEXPR: Hef1a-2x Kturn-eYFP
- with Nelson
Nelson is looking for itWill be propagated ASAP

 

EXPERIMENTS

  1. Hef1a eBFP2 & Hef1a 2x Kturn eYFP (positive control)
    1. Should be yellow and blue
  2. Hef1a eBFP2 & Hef1a 2x Kturn eYFP & Hef1a-L7ae (negative control)
    1. Should be blue (minimal yellow)

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titleTAL14
 
NameLocationConcentration
pEXPR: Hef1a-eBFP2
- in freezer
 
Our -20Currently being propagated
pEXPR: Hef1a-Gal4Vp16
- possibly with Jin? (get
With JinGetting in contact with
him)
Jin
  
pEXPR: Hef1a-TAL14
- Brian has, in pENTRY form
With BrianAwaiting to be LR'd with Hef1a
pEXPR: 5xUAS/4xTAL14bs-eYFP
- getting from Samira (but missing UAS)
With SamiraUAS needs to be added

 

EXPERIMENTS:

  1. Hef1a-eBFP2 & 5xUAS/4xTAL14bs-eYFP
    1. negative control (minimal yellow)
  2. Hef1a-eBFP2 & Hef1a-Gal4VP16 & 5xUAS/4xTAL14bs-eYFP
    1. positive control (hopefully linear BFP/YFP)
  3. Hef1a-eBFP2 & Hef1a-Gal4VP16 & Hef1a-TAL14 & 5xUAS/4xTAL14bs-eYFP
    1. Should be blue (minimal yellow)

 

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