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Comment: Task marked complete

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Cloak
22 complete 7/3: Seed cells 23 complete 7/4: Transfect hEF1a:eYFP and hEF1a:mKate into HEK cells 24 incomplete complete 7/6 (1pm): Flow Cytometer

 

Y1
  • hEF1a:eYFP
(Y+R)1
  • hEF1a:eYFP
  • hEF1a:mKate
 ////// ////// ////// -ve control
  • dummy DNA
  • with Lipofectamine
Y2
  • hEF1a:eYFP
(Y+R)2
  • hEF1a:eYFP
  • hEF1a:mKate
 ////// ////// ////// -ve control
  • dummy DNA
  • with Lipofectamine
R1
  • hEF1a:mKate
(Y+R)3
  • hEF1a:eYFP
  • hEF1a:mKate
 ////// ////// ////// -ve/-ve control
  • no DNA
  • no Lipofectamine
R2
  • hEF1a:mKate
(Y+R)4
  • hEF1a:eYFP
  • hEF1a:mKate
 ////// ////// ////// -ve/-ve control
  • no DNA
  • no Lipofectamine

...