Gel Extraction (QIAquick Extraction Kit protocol here updated with extra tips!)
Preparing the Gel
- Dissolve UltraPure agarose to a final concentration of 1%(by mass) in TAE buffer in a glass bottle.
- Heat the solution in the microwave with frequent stirring to dissolve the agarose homogenously. ~1 minute/200ml solution
- Place the solution in a warm water bath for 5 mins.
- Add 10 µl SYBRSafe (1:10000) per 100 ml of the solution and mix well.
- Pour 50ml of solution per small gel tray. (the gel trays and combs should be pre-cleaned with water and wiped dry).
- Note for combs: 15-well combs hold about 6 ul liquid, 12-well combs hold about 15 ul, 8-well combs hold about 20 ul
- Taping two 8-well comb wells together results in a well that holds up to 100 ul
- Taping three 8-well comb wells together result in a well that holds up to 200 ul
- Note for combs: 15-well combs hold about 6 ul liquid, 12-well combs hold about 15 ul, 8-well combs hold about 20 ul
- Use 120ml per large gel tray. [need to update amounts]
- For the small set: small trays hold 20ml, large trays hold 50ml
- Wait for the gels to solidify. ~15 mins
- Label and store at 4C.
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