Versions Compared

Old Version 40

changes.mady.by.user Unknown User (amgarcia@mit.edu)

Saved on

compared with

New Version 41

changes.mady.by.user Unknown User (amgarcia@mit.edu)

Saved on

Key

  • This line was added.
  • This line was removed.
  • Formatting was changed.

...

Toggle Cloak
PRACTICE #2: Co-transfection (2 fluorescent proteins under constitutive promoter)

Cloak
22 complete 7/23: Seed cells 23 incomplete 7/34: Transfect hEF1a:eYFP and hEF1a:mKate into HEK cells 24 incomplete 7/5 6 (__pm1pm): Flow Cytometer

 

Y1
  • hEF1a:eYFP
(Y+R)1
  • hEF1a:eYFP
  • hEF1a:mKate
 ////// ////// ////// -ve control
  • dummy DNA
  • with Lipofectamine
Y2
  • hEF1a:eYFP
(Y+R)2
  • hEF1a:eYFP
  • hEF1a:mKate
 ////// ////// ////// -ve control
  • dummy DNA
  • with Lipofectamine
R1
  • hEF1a:mKate
(Y+R)3
  • hEF1a:eYFP
  • hEF1a:mKate
 ////// ////// ////// -ve/-ve control
  • no DNA
  • no Lipofectamine
R2
  • hEF1a:mKate
(Y+R)4
  • hEF1a:eYFP
  • hEF1a:mKate
 ////// ////// ////// -ve/-ve control
  • no DNA
  • no Lipofectamine

...